Description
Description of exhibit and science, engineering, or technology that will be showcased:
For the Tandon Research Excellence Exhibit, we propose an interactive demonstration titled “Pollution Hunters: How DNA Reveals Hidden Contaminants.” This hands-on exhibit invites visitors to become environmental scientists and uncover invisible pollution in urban environment (Gowanus Canal) using DNA-based biosensors.
Participants will analyze simulated water samples through a mock LAMP-PCR test with pre-prepared, color-changing reactions: after adding a reaction mix to their sample tubes, a positive result turns bright pink, while a negative result stays yellow, simulating real-time detection of bacterial DNA linked to contamination. The demonstration is completely safe, using food-safe dyes to replicate the visual outcome of isothermal amplification.
To enrich the experience, visitors will also explore a 3D biosensor model that explains how genetic sequences and enzymes work together to detect microbes related to contamination, and they will perform a simple DNA extraction from strawberries, connecting the concept of environmental DNA (eDNA) to everyday life.
This exhibit combines bioinformatics, biotechnology, and design to show how innovative tools can monitor ecosystem health, offering visitors an engaging look at how science tracks pollution we can’t see.
🧪 Materials and Equipment List:
For Mock LAMP-PCR Color-Changing Demo:
- Pre-labeled microcentrifuge tubes (for mock “water samples”)
- Safe, food-grade color-changing solutions (e.g., pH indicator dyes like phenol red or bromothymol blue)
- Pipettes (P1000 and P200) and sterile disposable pipette tips
- Tube racks
- Disposable gloves
- Waste container for used tips and tubes
- Instruction cards or visual guide for the mock LAMP workflow
- Printed result key showing color interpretations (positive vs. negative)
For DNA Extraction Activity (Strawberry DNA):
- Fresh strawberries
- Ziplock bags
- Extraction buffer (dish soap + salt + water mixture)
- Cheesecloth or coffee filters
- Plastic funnels
- Test tubes or clear cups
- Cold isopropanol or ethanol (for DNA precipitation)
- Wooden sticks or disposable pipettes (to spool DNA)
- Waste disposal container
- Napkins or wipes for clean-up
For Biosensor Visualization:
- 3D-printed or handmade model of a biosensor (illustrating primers, enzymes, and detection process)
- Informational poster or diagram explaining how biosensors work in environmental monitoring
- Display stand for the biosensor model
For Digital or Visual Support:
- Tablet or laptop (optional) with animations or visualizations of microbial DNA detection
- Monitor or screen (optional) for larger visual display
- Power strip and extension cord (if electricity is needed)
General Exhibit Materials:
- Table covering
- Hand sanitizer
- Signage with exhibit title (“Pollution Hunters: How DNA Reveals Hidden Contaminants”)
- Name tags for exhibitors
- QR codes or flyers with links for visitors to learn more about the research